2R05

Crystal Structure of ALIX/AIP1 in complex with the HIV-1 YPLASL Late Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Wavelength(s)	1.00000
Spacegroup name	C 1 2 1
Unit cell lengths	146.090, 99.440, 73.543
Unit cell angles	90.00, 107.29, 90.00

Refinement procedure

Resolution	27.900 - 2.550
R-factor	0.228
Rwork	0.225
R-free	0.28000
RMSD bond length	0.013
RMSD bond angle	1.309
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.640
High resolution limit [Å]	2.550	5.490	2.550
Rmerge	0.126	0.141	0.366
Number of reflections	32602
<I/σ(I)>	19.7
Completeness [%]	98.6	87.5	99.5
Redundancy	5.7	5.4	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.9	286	0.20-0.25 M MgCl2, 7-10% PEG 4000, 0.1 M NaMES, pH 5.9, VAPOR DIFFUSION, SITTING DROP, temperature 286K