2R03

Crystal Structure of ALIX/AIP1 in complex with the YPDL Late Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Wavelength(s)	0.97946
Spacegroup name	C 1 2 1
Unit cell lengths	146.383, 98.570, 72.799
Unit cell angles	90.00, 107.07, 90.00

Refinement procedure

Resolution	50.000 - 2.590
R-factor	0.226
Rwork	0.223
R-free	0.28600
RMSD bond length	0.014
RMSD bond angle	1.384
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.690
High resolution limit [Å]	2.590	5.600	2.590
Rmerge	0.150	0.128	0.306
Number of reflections	29646
<I/σ(I)>	16.7
Completeness [%]	98.2	92	96.5
Redundancy	3	3	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.9	286	0.20-0.25 M MgCl2, 7-10% PEG 4000, 0.1 M NaMES, pH 5.9, VAPOR DIFFUSION, SITTING DROP, temperature 286K