2R02
Crystal Structure of ALIX/AIP1 in complex with the HIV-1 YPLTSL Late Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Wavelength(s) | 0.97946 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 145.923, 99.078, 73.226 |
Unit cell angles | 90.00, 107.32, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.600 |
R-factor | 0.228 |
Rwork | 0.224 |
R-free | 0.28500 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.511 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 5.600 | 2.600 |
Rmerge | 0.060 | 0.037 | 0.252 |
Number of reflections | 29846 | ||
<I/σ(I)> | 24 | ||
Completeness [%] | 98.4 | 92.2 | 94.2 |
Redundancy | 3.8 | 3.6 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | sitting drop vapor diffusion | 5.9 | 286 | 0.20-0.25 M MgCl2, 7-10% PEG 4000, 0.1 M NaMES, pH 5.9, sitting drop vapor diffusion, temperature 286K |