2QZW
Secreted aspartic proteinase (Sap) 1 from Candida albicans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-10 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.05 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 60.769, 135.668, 96.492 |
| Unit cell angles | 90.00, 96.74, 90.00 |
Refinement procedure
| Resolution | 19.900 - 2.050 |
| R-factor | 0.194 |
| Rwork | 0.189 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zap |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.368 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.051 | 0.100 |
| Number of reflections | 47773 | |
| <I/σ(I)> | 27.3 | |
| Completeness [%] | 98.4 | 99.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 294 | 14% PEG 20000, 0.1 M MES/NaOH, pH 6.3, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
