2QZT

Crystal Structure of Sterol Carrier Protein 2 Like 2 (SCP2-L2) from Aedes Aegypti

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	263
Detector technology	CCD
Collection date	2006-07-13
Detector	BRUKER PROTEUM
Spacegroup name	P 1 21 1
Unit cell lengths	45.360, 56.440, 50.610
Unit cell angles	90.00, 119.40, 90.00

Refinement procedure

Resolution	25.000 - 1.700
R-factor	0.2
Rwork	0.198
R-free	0.23600
Structure solution method	MIR
RMSD bond length	0.014
RMSD bond angle	1.425
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	24343
<I/σ(I)>	25.8	4.9
Completeness [%]	98.8	93.8
Redundancy	3.5	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	296	2 microl protein @ 10mg/ml was mixed with 2microl mother liquor consisting of 1.6 M sodium citrate, 5% glycerol, 100 mM Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K
1	VAPOR DIFFUSION, HANGING DROP	7.5	296	2 microl protein @ 10mg/ml was mixed with 2microl mother liquor consisting of 1.6 M sodium citrate, 5% glycerol, 100 mM Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K
1	VAPOR DIFFUSION, HANGING DROP	7.5	296	2 microl protein @ 10mg/ml was mixed with 2microl mother liquor consisting of 1.6 M sodium citrate, 5% glycerol, 100 mM Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K