2QXL

Crystal Structure Analysis of Sse1, a yeast Hsp110

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-03
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97923
Spacegroup name	C 1 2 1
Unit cell lengths	218.045, 125.331, 74.708
Unit cell angles	90.00, 98.67, 90.00

Refinement procedure

Resolution	29.490 - 2.410
R-factor	0.197
Rwork	0.195
R-free	0.23500
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.193
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.08)
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.490
High resolution limit [Å]	2.400	5.160	2.400
Rmerge	0.055	0.039	0.279
Number of reflections	75393
<I/σ(I)>	15.9
Completeness [%]	99.6	98.2	99.7
Redundancy	3.6	3.3	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	10% PEG 6000, 0.1M cacodylate, pH 6.5, temperature 293K, VAPOR DIFFUSION, HANGING DROP
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	10% PEG 6000, 0.1M cacodylate, pH 6.5, temperature 293K, VAPOR DIFFUSION, HANGING DROP