2QXF
Product bound structure of exonuclease I at 1.5 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-26 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.630, 91.849, 102.752 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.320 - 1.500 |
| R-factor | 0.20044 |
| Rwork | 0.199 |
| R-free | 0.22357 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.759 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR (2.5) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 3.230 | 1.500 |
| Rmerge | 0.040 | 0.022 | 0.371 |
| Number of reflections | 80289 | ||
| <I/σ(I)> | 16.2 | ||
| Completeness [%] | 99.8 | 98.6 | 99.7 |
| Redundancy | 6.8 | 6.8 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.2M Sodium chloride, 0.1M Tris-HCl, 20% PEG 8000, 1.5x molar excess ssDNA, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
