2QWR

Crystal structure of disulfide-bond-crosslinked complex of bovine hsc70 (1-394aa)R171C and bovine Auxilin (810-910aa)D876C in the AMPPNP intact form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Detector technology	IMAGE PLATE
Collection date	2007-04-05
Detector	RIGAKU RAXIS HTC
Spacegroup name	P 21 21 21
Unit cell lengths	38.797, 56.494, 225.878
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.670 - 2.210
R-factor	0.233
Rwork	0.230
R-free	0.29400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.537
Data reduction software	DENZO
Data scaling software	d*TREK
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.280
High resolution limit [Å]	2.200	4.740	2.200
Rmerge	0.072	0.039	0.556
Number of reflections	25924
<I/σ(I)>	10.6
Completeness [%]	99.8	98.9	100
Redundancy	3.3	3.2	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	microbatch under oil	8.5	289	PEG3350, Ammonium Acetate, pH 8.5, microbatch under oil, temperature 289K