2QWQ
Crystal structure of disulfide-bond-crosslinked complex of bovine hsc70 (1-394aa)R171C and bovine Auxilin (810-910aa)D876C in the AMPPNP hydrolyzed form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Detector technology | IMAGE PLATE |
Collection date | 2007-04-05 |
Detector | RIGAKU RAXIS HTC |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.797, 56.494, 225.878 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.670 - 2.210 |
R-factor | 0.243 |
Rwork | 0.240 |
R-free | 0.29500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.395 |
Data reduction software | DENZO |
Data scaling software | d*TREK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 4.740 | 2.200 |
Rmerge | 0.072 | 0.039 | 0.556 |
Number of reflections | 25924 | ||
<I/σ(I)> | 10.6 | ||
Completeness [%] | 99.8 | 98.9 | 100 |
Redundancy | 3.3 | 3.2 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | microbatch under oil | 8.5 | 289 | PEG3350, Ammonium Acetate, pH 8.5, microbatch under oil, temperature 289K |