2QW9
Crystal structure of bovine hsc70 (1-394aa)in the apo state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-07-24 |
| Detector | RIGAKU RAXIS HTC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.336, 78.236, 75.462 |
| Unit cell angles | 90.00, 100.81, 90.00 |
Refinement procedure
| Resolution | 35.090 - 1.850 |
| R-factor | 0.204 |
| Rwork | 0.202 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.232 |
| Data reduction software | DENZO |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.048 | 0.472 |
| Number of reflections | 70516 | |
| <I/σ(I)> | 13.3 | |
| Completeness [%] | 98.7 | 99.9 |
| Redundancy | 2.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch under oil | 8 | 289 | PEG3350, Calcium Acetate, pH 8.0, microbatch under oil, temperature 289K |
