2QVV

Porcine Liver Fructose-1,6-bisphosphatase cocrystallized with Fru-2,6-P2 and Zn2+, I(T)-state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-01-29
Detector	NOIR-1
Spacegroup name	P 21 21 2
Unit cell lengths	59.038, 165.709, 79.167
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.330 - 2.030
R-factor	0.201
Rwork	0.200
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ID 2QVU
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.080	2.130
High resolution limit [Å]	2.030	2.030
Rmerge	0.080	0.321
Total number of observations		37301
Number of reflections	51027
<I/σ(I)>	12	3.9
Completeness [%]	99.9	100
Redundancy	5.7	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	see citation, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K