2QV8
Structure of the minor pseudopilin EpsH from the Type 2 Secretion System of Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97903 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.349, 70.449, 85.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.171 |
| Rwork | 0.169 |
| R-free | 0.21200 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.537 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.060 |
| High resolution limit [Å] | 1.990 | 4.290 | 1.990 |
| Rmerge | 0.070 | 0.043 | 0.271 |
| Number of reflections | 21140 | ||
| <I/σ(I)> | 11.5 | ||
| Completeness [%] | 93.2 | 99.9 | 100 |
| Redundancy | 7.1 | 6.8 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.5 | 298 | PEG 4000, pH 4.5, vapor diffusion, temperature 298K |
