2QT7
Crystallographic structure of the mature ectodomain of the human receptor-type protein-tyrosine phosphatase IA-2 at 1.30 Angstroms
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-16 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9793, 1.5895 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.756, 66.799, 73.131 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.970 - 1.300 |
| R-factor | 0.22159 |
| Rwork | 0.220 |
| R-free | 0.24216 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.161 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.000 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.030 | 0.213 |
| Number of reflections | 38269 | |
| <I/σ(I)> | 43.1 | 5.5 |
| Completeness [%] | 97.7 | 81.4 |
| Redundancy | 7.6 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | 25% (w/v) PEG 4000, 0.1 M Tris pH 8.5, 0.2 M CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
