2QSW

Crystal structure of C-terminal domain of ABC transporter / ATP-binding protein from Enterococcus faecalis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2007-07-30
Detector	SBC-3
Wavelength(s)	0.97920
Spacegroup name	P 43 2 2
Unit cell lengths	38.896, 38.896, 111.938
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.800 - 1.500
R-factor	0.149
Rwork	0.147
R-free	0.18420
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	1.704
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	SHELXD
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.800	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.067	0.438
Number of reflections	14492
<I/σ(I)>	45.5	2.48
Completeness [%]	99.4	94.2
Redundancy	11.2	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.9	277	0.01 M Zinc sulfate, 0.1 M MES buffer pH 6.9, 25% PEG 550 MME, VAPOR DIFFUSION, SITTING DROP, temperature 277K