2QSS
Bovine hemoglobin at pH 6.3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2006-07-01 |
| Detector | BRUKER PROTEUM-R |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.999, 78.381, 109.247 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.630 - 1.750 |
| R-factor | 0.18 |
| Rwork | 0.177 |
| R-free | 0.22500 |
| Starting model (for MR) | 2qsp |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.380 |
| Data reduction software | SAINT |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.630 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 59622 | |
| Completeness [%] | 98.6 | 83.5 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 298 | 0.3 M Na Cacodylate 17% PEG 3350, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
