2QSP
Bovine Hemoglobin at pH 5.7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2006-06-15 |
| Detector | BRUKER PROTEUM-R |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.033, 78.273, 109.085 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.630 - 1.850 |
| R-factor | 0.19027 |
| Rwork | 0.187 |
| R-free | 0.25768 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g0a |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.525 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.630 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 52808 | |
| Completeness [%] | 99.6 | |
| Redundancy | 7.4 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 298 | 0.4 M Na Cacodylate 12-15% PEG 3350, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
