2QSI
Crystal structure of putative hydrogenase expression/formation protein hupG from Rhodopseudomonas palustris CGA009
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97940 |
| Spacegroup name | P 1 |
| Unit cell lengths | 31.575, 41.126, 46.850 |
| Unit cell angles | 107.72, 99.85, 101.75 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.15455 |
| Rwork | 0.152 |
| R-free | 0.20133 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.058 | 0.124 |
| Number of reflections | 17173 | |
| <I/σ(I)> | 14.4 | 7.8 |
| Completeness [%] | 87.2 | |
| Redundancy | 2.7 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 291 | 1.5M Ammonium sulfate, 50mM MES pH 5.6, 2% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
