2QS1
Crystal structure of the GluR5 ligand binding core dimer in complex with UBP315 at 1.80 Angstroms resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-12-02 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 97.823, 97.816, 129.043 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.570 - 1.800 |
| R-factor | 0.19864 |
| Rwork | 0.198 |
| R-free | 0.21916 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2f34 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.331 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.040 | 0.273 |
| Number of reflections | 57347 | |
| <I/σ(I)> | 20.9 | 4.73 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.9 | 293 | 18% PEG 1K, 100 mM Tris-Cl, 2.5 mM UBP315, pH 8.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
