2QRX
Crystal structure of Drosophila melanogaster Translin protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-09-19 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 152.778, 152.778, 94.007 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.600 |
| R-factor | 0.217 |
| Rwork | 0.215 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j1j |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.804 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.790 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.106 | 0.412 |
| Number of reflections | 7311 | |
| <I/σ(I)> | 13.3 | 2.8 |
| Completeness [%] | 93.7 | 91.4 |
| Redundancy | 2.8 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 2.0M ammonium sulfate, 2% MPD, 5% glycerol, 100mM MES buffer, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
