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2QRC

Crystal structure of the adenylate sensor from AMP-activated protein kinase in complex with ADP and AMP

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 24-ID-C
Synchrotron siteAPS
Beamline24-ID-C
Temperature [K]100
Detector technologyCCD
Collection date2007-02-15
DetectorADSC QUANTUM 315
Wavelength(s)0.97926
Spacegroup nameC 1 2 1
Unit cell lengths168.293, 78.087, 108.553
Unit cell angles90.00, 124.13, 90.00
Refinement procedure
Resolution48.280 - 2.700
R-factor0.2106
Rwork0.206
R-free0.28945
Structure solution methodFOURIER SYNTHESIS
Starting model (for MR)2ooy
RMSD bond length0.015
RMSD bond angle1.609
Data reduction softwareHKL-2000
Data scaling softwareSCALEPACK
Phasing softwareCNS
Refinement softwareREFMAC (5.2.0019)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.800
High resolution limit [Å]2.7002.700
Rmerge0.1070.472
Number of reflections30918
<I/σ(I)>7.41.5
Completeness [%]96.881.6
Redundancy3.42.4
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP5.52936-10% PEG 3350, 0.1M Sodium Citrate, pH 5.5, 5mM ADP, 5mM AMP, VAPOR DIFFUSION, HANGING DROP, temperature 293K

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