2QR7
2.0A X-ray structure of C-terminal kinase domain of p90 ribosomal S6 kinase 2: Se-Met derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97936 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 46.585, 46.585, 293.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.2045 |
| Rwork | 0.203 |
| R-free | 0.23776 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.217 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.060 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.079 | 0.440 |
| Number of reflections | 23160 | |
| <I/σ(I)> | 36.84 | 6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.7 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 7.5%-10% PEG3350, 50mM Ammonium sulfate, 0.1M Hepes, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
