2QR4
Crystal structure of oligoendopeptidase-F from Enterococcus faecium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 133.142, 133.142, 171.619 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.040 - 2.500 |
| R-factor | 0.191 |
| Rwork | 0.188 |
| R-free | 0.24400 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.142 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
| Rmerge | 0.102 | 0.055 | 0.764 |
| Number of reflections | 53824 | ||
| <I/σ(I)> | 6.4 | ||
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 15.8 | 15.1 | 15.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 0.1M Sodium acetate pH 4.6, 1.0M Sodium formate, VAPOR DIFFUSION, SITTING DROP |
