2QR0
Structure of VEGF complexed to a Fab containing TYR and SER in the CDRs
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 154.453, 90.218, 205.558 |
| Unit cell angles | 90.00, 90.85, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.500 |
| R-factor | 0.292 |
| Rwork | 0.291 |
| R-free | 0.31200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.037 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.630 |
| High resolution limit [Å] | 3.500 | 7.530 | 3.500 |
| Rmerge | 0.097 | 0.037 | 0.444 |
| Number of reflections | 71940 | ||
| <I/σ(I)> | 10.7 | ||
| Completeness [%] | 96.1 | 99.4 | 93.4 |
| Redundancy | 3.3 | 3.6 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
