2QQS
JMJD2A tandem tudor domains in complex with a trimethylated histone H4-K20 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.970, 27.689, 82.424 |
| Unit cell angles | 90.00, 90.77, 90.00 |
Refinement procedure
| Resolution | 25.970 - 2.820 |
| R-factor | 0.23811 |
| Rwork | 0.236 |
| R-free | 0.28292 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qqr |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.559 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.920 |
| High resolution limit [Å] | 2.820 | 2.820 |
| Rmerge | 0.107 | 0.396 |
| Number of reflections | 5184 | |
| <I/σ(I)> | 16.2 | 1.2 |
| Completeness [%] | 88.8 | |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 293 | 293K |
