2QPO
Thermotoga Maritima Thymidine Kinase in the apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-09-26 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.600, 113.500, 54.800 |
| Unit cell angles | 90.00, 109.16, 90.00 |
Refinement procedure
| Resolution | 28.610 - 1.950 |
| R-factor | 0.20899 |
| Rwork | 0.203 |
| R-free | 0.26115 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.594 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.610 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 43333 | |
| <I/σ(I)> | 13.8 | 4.4 |
| Completeness [%] | 98.0 | 75 |
| Redundancy | 3.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 20% PEG 3350 + 0.2 M NaSO4, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
