2QP9

Crystal Structure of S.cerevisiae Vps4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Detector technology	CCD
Spacegroup name	P 65 2 2
Unit cell lengths	86.553, 86.553, 236.061
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.900
R-factor	0.25996
Rwork	0.258
R-free	0.28819
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.137
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.063	0.606
Number of reflections	12599
<I/σ(I)>	37.5	3.8
Completeness [%]	98.1	99.2
Redundancy	13.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	Sodium Acetate, CdSO4, pH 6.5, temperature 293K, VAPOR DIFFUSION
1	VAPOR DIFFUSION	6.5	293	Sodium Acetate, CdSO4, pH 6.5, temperature 293K, VAPOR DIFFUSION
1	VAPOR DIFFUSION	6.5	293	Sodium Acetate, CdSO4, pH 6.5, temperature 293K, VAPOR DIFFUSION