2QNJ
Kinase and Ubiquitin-associated domains of MARK3/Par-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-01 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97899 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.675, 112.544, 117.206 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.700 |
| R-factor | 0.21859 |
| Rwork | 0.215 |
| R-free | 0.27926 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zmu |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.646 |
| Data reduction software | DENZO |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmerge | 0.059 | 0.395 |
| Number of reflections | 23590 | |
| <I/σ(I)> | 13.6 | 3.3 |
| Completeness [%] | 99.4 | 95.5 |
| Redundancy | 6.4 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 1.5M LiSO4, 100 mM HEPES pH7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
