2QMW
The crystal structure of the prephenate dehydratase (PDT) from Staphylococcus aureus subsp. aureus Mu50
Replaces: 2IQ8Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97929, 0.97942 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 60.859, 87.406, 107.744 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.880 - 2.300 |
| R-factor | 0.22734 |
| Rwork | 0.225 |
| R-free | 0.27610 |
| Structure solution method | MAD |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.114 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.880 | 2.350 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.068 | 0.439 |
| Number of reflections | 25930 | |
| <I/σ(I)> | 56.5 | 5.5 |
| Completeness [%] | 98.4 | 97.8 |
| Redundancy | 11.1 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 289 | 0.1M Sodium citrate, 3M Sodium chloride, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
