2QMQ
Crystal structure of a n-myc downstream regulated 2 protein (ndrg2, syld, ndr2, ai182517, au040374) from mus musculus at 1.70 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-12 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 46.371, 46.371, 214.749 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.348 - 1.700 |
| R-factor | 0.147 |
| Rwork | 0.145 |
| R-free | 0.18300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u2e |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.561 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.348 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.050 | 0.405 |
| Number of reflections | 28650 | |
| <I/σ(I)> | 11.7 | 1.9 |
| Completeness [%] | 92.9 | 61.8 |
| Redundancy | 6.2 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 277 | NANODROP, 20.0% PEG 400, 0.2M MgCl2, 0.1M HEPES pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K, pH 7.50 |
