2QLP
Bifunctional dCTP deaminase:dUTPase from Mycobacterium tuberculosis, apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-22 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.800, 96.700, 78.000 |
| Unit cell angles | 90.00, 97.70, 90.00 |
Refinement procedure
| Resolution | 19.900 - 2.470 |
| R-factor | 0.232 |
| Rwork | 0.228 |
| R-free | 0.29500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.497 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 2.470 | 2.470 |
| Rmerge | 0.193 | 0.551 |
| Number of reflections | 32512 | |
| <I/σ(I)> | 6.81 | 2.2 |
| Completeness [%] | 99.1 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 1.9mg/ml enzyme in 50mM HEPES pH 6.8 Reservoir: 20% PEG 8K, 50mM MgCl2, 100mM HEPES pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
