2QLN

Glycogen Phosphorylase b in complex with N-4-phenylbenzoyl-N'-beta-D-glucopyranosyl urea

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	293
Detector technology	CCD
Collection date	2004-11-20
Detector	MAR CCD 165 mm
Wavelength(s)	0.813
Spacegroup name	P 43 21 2
Unit cell lengths	128.763, 128.763, 116.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.150
R-factor	0.18223
Rwork	0.181
R-free	0.20947
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	2prj
RMSD bond length	0.007
RMSD bond angle	1.043
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.190
High resolution limit [Å]	2.150	2.150
Number of reflections	53808
<I/σ(I)>	18.1	6.2
Completeness [%]	99.8	99.6
Redundancy	7.4	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	6.7	289	10 mM Bes, 3 mM DDT, pH 6.7, SMALL TUBES, temperature 289K