2QLK

Adenovirus AD35 fibre head

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-01-01
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	H 3
Unit cell lengths	87.831, 87.831, 53.897
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.400 - 2.020
R-factor	0.216
Rwork	0.200
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h7z
RMSD bond length	0.010
RMSD bond angle	1.550
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR (2.5)
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.090
High resolution limit [Å]	2.020	4.350	2.020
Rmerge	0.082	0.043	0.501
Number of reflections	9568
<I/σ(I)>	9.4
Completeness [%]	92.8	100	58
Redundancy	4.7	5.4	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	294	Protein in 20mM Tris-HCl, 200mM NaCl, 5mM DTT, reservoir: 30% PEG1000, 0.2M MgBr2, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K
1	VAPOR DIFFUSION, HANGING DROP	8	294	Protein in 20mM Tris-HCl, 200mM NaCl, 5mM DTT, reservoir: 30% PEG1000, 0.2M MgBr2, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K