2QL6
human nicotinamide riboside kinase (NRK1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0808 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 145.710, 99.960, 145.590 |
| Unit cell angles | 90.00, 91.57, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.700 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO (package) |
| Data scaling software | SCALEPACK (package) |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.095 | 0.280 |
| Number of reflections | 116539 | |
| <I/σ(I)> | 13.2 | 2.98 |
| Completeness [%] | 94.9 | 74.3 |
| Redundancy | 3.1 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 298 | 8% (w/v) PEG 3350, 200 mM NH4Cl, 5 mM DTT, 5 mM Na2HPO, pH 7.8, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
