2QKS

Crystal structure of a Kir3.1-prokaryotic Kir channel chimera

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-08-02
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1000
Spacegroup name	P 4
Unit cell lengths	98.416, 98.416, 92.624
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.500 - 2.200
R-factor	0.232
Rwork	0.231
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1N9P 1p7b
RMSD bond length	0.008
RMSD bond angle	1.280
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	43991
<I/σ(I)>	17	3.9
Completeness [%]	97.9	99.7
Redundancy	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	293	10-15% (w/v) PEG 4000, 0.05M sodium citrate, 0.2M potassium phosphate, 0.08M bis-tris, 0.12M KCl, 0.003M dithiothreitol, 0.02M tris (2-carboxyethyl) phosphine hydrochloride, 0.0026M 1,2-dihexanoyl phosphatidylinositol 4,5-biphosphate, 0.016M nonylglucoside , pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K