2QKO
Crystal structure of transcriptional regulator RHA06399 from Rhodococcus sp. RHA1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-14 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97929 |
Spacegroup name | P 31 |
Unit cell lengths | 62.307, 62.307, 164.243 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.430 - 2.350 |
R-factor | 0.23513 |
Rwork | 0.233 |
R-free | 0.27330 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.251 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.370 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.071 | 0.397 |
Number of reflections | 29688 | |
<I/σ(I)> | 41.24 | 4.29 |
Completeness [%] | 99.8 | 100 |
Redundancy | 5.7 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 289 | 0.1M Bis-tris, 2.5M Ammonium sulfate, 1,5-Diaminopentane di-HCl, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 289K |