2QK2

Structural Basis of Microtubule Plus End Tracking by XMAP215, CLIP-170 and EB1

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-02-28
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97963, 0.97974, 1.00801
Spacegroup name	C 2 2 21
Unit cell lengths	91.370, 113.414, 78.556
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.580 - 2.100
Rwork	0.202
R-free	0.21700
Structure solution method	MAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.035	0.185
Number of reflections	45149
<I/σ(I)>	17.9	4.3
Completeness [%]	98.2	91.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	2.25 M Sodium malonate, Protein concentration 15 mg/ml , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7	293	2.25 M Sodium malonate, Protein concentration 15 mg/ml , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7	293	2.25 M Sodium malonate, Protein concentration 15 mg/ml , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K