2QK1

Structural Basis of Microtubule Plus End Tracking by XMAP215, CLIP-170 and EB1

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-03-13
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97959, 0.97972, 1.01627, 1.12714
Spacegroup name	P 21 21 21
Unit cell lengths	28.441, 80.649, 97.919
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.850 - 1.700
Rwork	0.173
R-free	0.19600
Structure solution method	MAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.040	0.097
Number of reflections	25288
<I/σ(I)>	25.4	9.4
Completeness [%]	99.0	94.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	27% PEG 4000, 100 mM Sodium citrate, 200 mM Ammonium acetate, Protein concentration 15 mg/ml, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	27% PEG 4000, 100 mM Sodium citrate, 200 mM Ammonium acetate, Protein concentration 15 mg/ml, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	27% PEG 4000, 100 mM Sodium citrate, 200 mM Ammonium acetate, Protein concentration 15 mg/ml, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K