2QJX

Structural Basis of Microtubule Plus End Tracking by XMAP215, CLIP-170 and EB1

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-02-28
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97962, 0.97979, 1.03320
Spacegroup name	P 21 21 2
Unit cell lengths	35.849, 102.502, 31.334
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.840 - 1.900
Rwork	0.193
R-free	0.22500
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.155
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.034	0.068
Number of reflections	17245
<I/σ(I)>	35.4	12.4
Completeness [%]	98.2	85.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	100 mM Tris, 200 mM Lithium sulfate, 29% PEG 4000, Protein stock 15 mg/ml, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	9	293	100 mM Tris, 200 mM Lithium sulfate, 29% PEG 4000, Protein stock 15 mg/ml, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	9	293	100 mM Tris, 200 mM Lithium sulfate, 29% PEG 4000, Protein stock 15 mg/ml, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K