2QJL
Crystal structure of Urm1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 50.384, 63.009, 28.893 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.440 |
| R-factor | 0.16771 |
| Rwork | 0.166 |
| R-free | 0.19752 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ax5 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | AUTOMAR |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.470 |
| High resolution limit [Å] | 1.440 | 1.440 |
| Rmerge | 0.028 | 0.154 |
| Number of reflections | 15800 | |
| <I/σ(I)> | 13.3 | 4.2 |
| Completeness [%] | 96.3 | 91.4 |
| Redundancy | 8.51 | 7.08 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 0.2M MgCl2, 0.1M Tris, 30% PEG4000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
