2QJB

Crystal structure analysis of BMP-2 in complex with BMPR-IA variant IA/IB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-05-04
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	P 65
Unit cell lengths	104.175, 104.175, 100.012
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.270 - 2.500
R-factor	0.20682
Rwork	0.204
R-free	0.26259
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rew
RMSD bond length	0.014
RMSD bond angle	1.567
Data reduction software	CrystalClear
Data scaling software	CrystalClear
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.270	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.083	0.564
Number of reflections	21360
<I/σ(I)>	11.2	2.8
Completeness [%]	99.9	99.9
Redundancy	5.23	5.32

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	298	0.8M K Na phosphate, 25% ethylene glycol, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K