2QJA
Crystal structure analysis of BMP-2 in complex with BMPR-IA variant B12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 65 |
| Unit cell lengths | 105.740, 105.740, 97.610 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.580 - 2.600 |
| R-factor | 0.21569 |
| Rwork | 0.214 |
| R-free | 0.25409 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rew |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.900 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.610 | 2.650 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.062 | 0.499 |
| Number of reflections | 19967 | |
| <I/σ(I)> | 10.5 | 2.4 |
| Completeness [%] | 98.9 | 90.9 |
| Redundancy | 4.22 | 3.31 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | 0.8M K Na phosphate, 25% ethylene glycol, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
