2QIM
Crystal Structure of Pathogenesis-related Protein LlPR-10.2B from yellow lupine in complex with Cytokinin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-23 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8030 |
| Spacegroup name | P 65 |
| Unit cell lengths | 73.822, 73.822, 67.212 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 1.350 |
| R-factor | 0.14843 |
| Rwork | 0.147 |
| R-free | 0.19438 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | LlPR-10.2F |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.043 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.400 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.042 | 0.670 |
| Number of reflections | 45644 | |
| <I/σ(I)> | 56.7 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.7 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 1.2 M sodium citrate, 0.1 M MES pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
