2QIB
Crystal structure of tetR-family transcriptional regulator from Streptomyces coelicolor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97940 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.607, 76.292, 65.262 |
| Unit cell angles | 90.00, 107.54, 90.00 |
Refinement procedure
| Resolution | 62.260 - 1.700 |
| R-factor | 0.17921 |
| Rwork | 0.177 |
| R-free | 0.21412 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.374 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.600 | 1.743 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.094 | 0.624 |
| Number of reflections | 52953 | |
| <I/σ(I)> | 24.64 | 1.79 |
| Completeness [%] | 99.1 | 91.05 |
| Redundancy | 9 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2M KH2PO4, 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
