2QI9
ABC-transporter BtuCD in complex with its periplasmic binding protein BtuF
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Collection date | 2007-02-18 |
| Wavelength(s) | 0.93209, 0.97934, 0.97980 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 213.582, 127.396, 97.547 |
| Unit cell angles | 90.00, 112.76, 90.00 |
Refinement procedure
| Resolution | 29.814 - 2.600 |
| Rwork | 0.262 |
| R-free | 0.28000 |
| Structure solution method | MAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.814 | 2.690 |
| High resolution limit [Å] | 2.586 | 2.600 |
| Rmerge | 0.074 | 0.570 |
| Number of reflections | 74100 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.4 | 293 | 30-38% (w/v) polyethylene glycol 400, 50mM either of Tris‑HCl pH 8.4 or Glycine-NaOH pH 9.4 and 400mM (NH4)2SO4., VAPOR DIFFUSION, SITTING DROP, temperature 293K |
