2QHK
Crystal structure of methyl-accepting chemotaxis protein from Vibrio parahaemolyticus RIMD 2210633
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97940 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 112.268, 112.268, 62.731 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 79.310 - 1.910 |
| R-factor | 0.17369 |
| Rwork | 0.172 |
| R-free | 0.21274 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.342 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.310 | 1.957 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.133 | 0.620 |
| Number of reflections | 15013 | |
| <I/σ(I)> | 27 | 5 |
| Completeness [%] | 99.3 | 0.934 |
| Redundancy | 18.5 | 17 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1M Bis-Tris, 28% PEG MME 2000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
