2QGY
Crystal structure of an enolase from the environmental genome shotgun sequencing of the Sargasso Sea
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-24 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97958 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 88.636, 139.168, 121.153 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.800 |
R-factor | 0.178 |
Rwork | 0.175 |
R-free | 0.22200 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.415 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELXCD |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 74.760 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.104 | 0.634 |
Number of reflections | 69393 | |
<I/σ(I)> | 13.7 | 3.1 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 7.3 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.2 | 294 | 100 mM MES pH 6.2, 25% PEG 3350, 200 mM Magnesium chloride, VAPOR DIFFUSION, temperature 294K |