2QGO
Crystal structure of a putative Fe-S biosynthesis protein from Lactobacillus acidophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-19 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 41 |
Unit cell lengths | 61.261, 61.261, 40.442 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.560 - 2.040 |
R-factor | 0.226 |
Rwork | 0.226 |
R-free | 0.26700 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.110 |
High resolution limit [Å] | 2.040 | 2.040 |
Rmerge | 0.050 | 0.213 |
Number of reflections | 17922 | |
<I/σ(I)> | 11.4 | 2 |
Completeness [%] | 96.3 | 73 |
Redundancy | 6.5 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 298 | 1.8M Ammonium citrate, pH 7.0, temperature 298K |