2QGN

Crystal structure of tRNA isopentenylpyrophosphate transferase (BH2366) from Bacillus halodurans, Northeast Structural Genomics Consortium target BhR41.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-05-02
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97930
Spacegroup name	P 61
Unit cell lengths	50.855, 50.855, 204.697
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.990 - 2.400
R-factor	0.225
Rwork	0.225
R-free	0.27700
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SnB
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.990	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.103	0.391
Number of reflections	21615
<I/σ(I)>	18.3	4.09
Completeness [%]	94.8	100
Redundancy	8.9	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH UNDER OIL	7.5	291	Protein solution: 10 mM Tris-HCl pH 7.5, 100 mM NaCl, 20 micro-g/ml Subtilisin, 5 mM DTT. Reservoir solution: 16% PEG 3350, 200 mM Ammonium tartrate, MICROBATCH UNDER OIL, temperature 291K