2QGF

Structure of regulatory chain mutant H20A of asparate transcarbamoylase from E. coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1996-11-18
Detector	UCSD MARK II
Wavelength(s)	1.5418
Spacegroup name	P 3 2 1
Unit cell lengths	122.280, 122.280, 142.490
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.000 - 2.200
Rwork	0.197
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nbe
RMSD bond length	0.014
RMSD bond angle	1.700
Data reduction software	SDMS
Data scaling software	SDMS
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	35.000
High resolution limit [Å]	2.200
Rmerge	0.099
Number of reflections	59318
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	5.7	298	0.1M citric acid, pH 5.7, MICRODIALYSIS, temperature 298.0K