2QG7
Plasmodium vivax ethanolamine kinase Pv091845
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-BM |
| Synchrotron site | APS |
| Beamline | 17-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-10 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.031, 172.702, 93.353 |
| Unit cell angles | 90.00, 110.72, 90.00 |
Refinement procedure
| Resolution | 24.930 - 2.407 |
| R-factor | 0.2331 |
| Rwork | 0.230 |
| R-free | 0.28974 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ck0 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.342 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 85067 | |
| <I/σ(I)> | 20.28 | 2 |
| Completeness [%] | 95.1 | 57.4 |
| Redundancy | 3.4 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 1.4M Na Citrate, 0.1M Hepes pH7.5, 2mM TCEP, 1mM MgCl2, 4mm CaCl2, 4mM ethanolamine chloride, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
